Antibacterial and antibiofilm activities of taxifolin against vancomycin-resistant S. aureus (VRSA)


  • Nisreen A. Abid Department of Microbiology, College of Medicine, University of Karbala, Karbala, Iraq
  • Entisar M. Hamad Department of Nursing, Technical Institute of Baquba, Middle Technical University, Baqubah 32001, Iraq
  • Musaab A. Ibrahim Baquba Teaching Hospital, Iraqi Ministry of Health, Baqubah 32001, Iraq
  • Hussein Abid Medical Laboratory Technology Department, Technical Institute of Baquba, Middle Technical University, Baqubah 32001, Iraq



antibacterial, biofilm, resazurin, Staphylococcus aureus, taxifolin


Background and objective: The medicinal effects of flavonoids are widely described in the literature; however, their antimicrobial effects against antibiotic resistant bacteria are yet to be highlighted. This study was aimed at investigating the growth and biofilm inhibitory effects of taxifolin, a flavonoid, against vancomycin-resistant Staphylococcus aureus (VRSA).

Methods: Seven VRSA isolates were used to assess the antimicrobial and antibiofilm influence of taxifolin. The agar-well diffusion method was used to determine the zones of inhibition caused by taxifolin, and resazurin-based microdilution technique was used to assess the minimum inhibitory concentration. Crystal violet staining technique was used to assess the biomass of biofilms formed by the microorganisms. GraphPad Prism software was used to present the data in figures.

Results: Taxifolin inhibited bacterial growth in a dose-dependent fashion and reduced bacterial viability. It similarly attenuated the biofilm production activity of bacterial isolates in a dose-dependent manner.

Conclusions: Current findings suggest the antibacterial and antibiofilm influence of taxifolin against VRSA in a dose-dependent manner.


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A Pardede and M Koketsu. “Antioxidant and antileukemic activity of chemical components from bark of Mangifera casturi”. Comp Clin Pathol 26 (2017), pp. 499–504. DOI: 10.1007/s00580-016-2387-x. DOI:

Clara Grosso et al. “Essential oil composition of Pterospartum tridentatum grown in Portugal”. Food Chem 102(4) (2007), pp. 1083–1088. DOI: 10.1016/j.foodchem.2006.06.049. DOI:

Alaguvel Valliammai et al. “5-Dodecanolide interferes with biofilm formation and reduces the virulence of Methicillin-resistant Staphylococcus aureus (MRSA) through up regulation of agr system”. Sci Rep 9(1) (2019), pp. 13744–13744. DOI: 10.1038/s41598-019-50207-y. DOI:

Alfredo Aires et al. “Phytochemical composition and antibacterial activity of hydroalcoholic extracts of Pterospartum tridentatum and Mentha pulegium against Staphylococcus aureus isolates”. Biomed Res Int 2016 (2016), pp. 5201879–5201879. DOI: 10.1155/2016/5201879. DOI:

Anita Elaine Weidmann. “Dihydroquercetin: More than just an impurity?” Eur J Pharmacol 684(1-3) (2012), pp. 19–26. DOI: 10.1016/j.ejphar.2012.03.035. DOI:

Bilal Aslam et al. “Antibiotic resistance: a rundown of a global crisis”. Infect Drug Resist 11 (2018), pp. 1645–1658. DOI: 10.2147/IDR.S173867. DOI:

Christudas Sunil and Baojun Xu. “An insight into the health-promoting effects of taxifolin (dihydroquercetin)”. Phytochemistry 166 (2019), pp. 112066–112066. DOI: 10.1016/j.phytochem.2019.112066. DOI:

Cong Cai et al. “Effects of taxifolin on osteoclastogenesis in vitro and in vivo”. Front Pharmacol 9 (2018), pp. 1–10. DOI: 10.3389/fphar.2018.01286. DOI:

Cristina D Cruz, Shreya Shah, and Päivi Tammela. “Defining conditions for biofilm inhibition and eradication assays for Gram-positive clinical reference strains”. BMC Microbiol 18(1) (2018), pp. 173–173. DOI: 10.1186/s12866-018-1321-6. DOI:

Daniel A. Abugri et al. “In vitro activity of the interaction between taxifolin (dihydroquercetin) and pyrimethamine against Toxoplasma gondii”. Chem Biol Drug Des 91(1) (2018), pp. 194–201. DOI: 10.1111/cbdd.13070. DOI:

Fabíola Carina Biluca et al. “Phenolic compounds, antioxidant capacity and bioaccessibility of minerals of stingless bee honey (Meliponinae)”. J Food Compost Anal 63 (2017), pp. 89–97. DOI: 10.1016/j.jfca.2017.07.039. DOI:

Faegheh Farhadi et al. “Antibacterial activity of flavonoids and their structure-activity relationship: An update review”. Phytother Res 33(1) (2019), pp. 13–40. DOI: 10.1002/ptr.6208. DOI:

Fevzi Topal et al. “Antioxidant activity of taxifolin: an activity-structure relationship”. J Enzyme Inhib Med Chem 31(4) (2016), pp. 674–683. DOI: 10.3109/14756366.2015.1057723. DOI:

Harlinda Kuspradini, Tohru Mitsunaga, and Hideo Ohashi. “Antimicrobial activity against Streptococcus sobrinus and glucosyltransferase inhibitory activity of taxifolin and some flavanonol rhamnosides from kempas (Koompassia malaccensis) extracts”. J Wood Sci 55 (), pp. 308–313. DOI: 10.1007/s10086-009-1026-4. DOI:

Ishaku Leo Elisha et al. “The antibacterial activity of extracts of nine plant species with good activity against Escherichia coli against five other bacteria and cytotoxicity of extracts”. BMC Complement Altern Med 17 (2017), pp. 133–133. DOI: 10.1186/s12906-017-1645-z. DOI:

J An et al. “Antibacterial and synergy of a flavanonol rhamnoside with antibiotics against clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA)”. Phytomedicine 18(11) (2011), pp. 990–993. DOI: 10.1016/j.phymed.2011.02.013. DOI:

J B Harborne and C A Williams. “Advances in flavonoid research since 1992”. Phytochemistry 55(6) (2000), pp. 481–504. DOI: 10.1016/s0031-9422(00)00235-1.

Jiajie Chen et al. “Pretreatment with dihydroquercetin, a dietary flavonoid, protected against concanavalin A-induced immunological hepatic injury in mice and TNF-α/ActD-induced apoptosis in HepG2 cells”. Food Funct 9(4) (2018), pp. 2341–2352. DOI: 10.1039/c7fo01073g. DOI:

Jing Tang et al. “The use of vancomycin in the treatment of adult patients with methicillin-resistant Staphylococcus aureus (MRSA) infection: a survey in a tertiary hospital in China”. Int J Clin Exp Med 8(10) (2015), pp. 19436–19441.

John V. Bennett et al. “Simplified, accurate method for antibiotic assay of clinical specimens”. Appl Microbiol 14(2) (), pp. 170–177. DOI: 10.1128/am.14.2.170-177.1966. DOI:

K. Morteza-Semnani, M. Saeedi, and Mohammad Akbarzadeh. “Chemical composition and antimicrobial activity of the essential oil of mentha pulegium L”. J Essent Oil Bear Pl 14(2) (2011), pp. 208–213. DOI: 10.1080/0972060X.2011.10643923. DOI:

Krishnan Manigandan et al. “Taxifolin curbs NF-κB-mediated Wnt/β-catenin signaling via up-regulating Nrf2 pathway in experimental colon carcinogenesis”. Biochimie 119 (2015), pp. 103–112. DOI: 10.1016/j.biochi.2015.10.014. DOI:

Laênia Angélica Andrade Lopes et al. “Inhibitory effects of flavonoids on biofilm formation by Staphylococcus aureus that overexpresses efflux protein genes”. Microb Pathog 107 (2017), pp. 193–197. DOI: 10.1016/j.micpath.2017.03.033. DOI:

Leticia Matilla-Cuenca et al. “Antibiofilm activity of flavonoids on staphylococcal biofilms through targeting BAP amyloids”. Sci Rep 10(1) (2020), pp. 18968–18968. DOI: 10.1038/s41598-020-75929-2. DOI:

M C Ploy et al. “First clinical isolate of vancomycin-intermediate Staphylococcus aureus in a French hospital”. Lancet 351(9110) (1998), pp. 1212–1212. DOI: 10.1016/s0140-6736(05)79166-2. DOI:

Mohaddese Mahboubi and Ghasem Haghi. “Antimicrobial activity and chemical composition of Mentha pulegium L. essential oil”. J Ethnopharmacol 119(2) (2008), pp. 325–327. DOI: 10.1016/j.jep.2008.07.023. DOI:

Mohamed Elshikh et al. “Resazurin-based 96-well plate microdilution method for the determination of minimum inhibitory concentration of biosurfactants”. Biotechnol Lett 38(6) (2016), pp. 1015–1019. DOI: 10.1007/s10529-016-2079-2. DOI:

Nabaa A. Jasima et al. “ZnO nanoparticles inhibit growth and biofilm formation of vancomycin-resistant S. aureus (VRSA)”. Biocatal Agri Biotechnol 29 (2020), pp. 101745–101745. DOI: 10.1016/j.bcab.2020.101745. DOI:

Patrudu S Makena and King-Thom Chung. “Effects of various plant polyphenols on bladder carcinogen benzidine-induced mutagenicity”. Food Chem Toxicol 75(10) (2007), pp. 1899–1909. DOI: 10.1016/j.fct.2007.04.007. DOI:

Ronghua Wang et al. “The anti-tumor effect of taxifolin on lung cancer via suppressing stemness and epithelial-mesenchymal transition in vitro and oncogenesis in nude mice”. Ann Transl Med 8(9) (2020), pp. 590–590. DOI: 10.21037/atm-20-3329. DOI:

S Stepanovic et al. “A modified microtiter-plate test for quantification of staphylococcal biofilm formation”. J Microbiol Methods 40(2) (2000), pp. 175–179. DOI: 10.1016/s0167-7012(00)00122-6. DOI:

S Thanish Ahamed and T Lakshmi. “Antibacterial activity of taxifolin isolated from Acacia catechu leaf extract-An in vitro study”. Int J Pharm Res Allied Sci 7(4) (2018), pp. 133–137.

Saet Byoul Lee et al. “The chemopreventive effect of taxifolin is exerted through ARE-dependent gene regulation”. Biol Pharm Bull 30(6) (2007), pp. 1074–1079. DOI: 10.1248/bpb.30.1074. DOI:

Sameer Mohammed Yaseen et al. “Antibacterial activity of palm heart extracts collected from Iraqi Phoenix dactylifera L.” J Tech 1(1) (2019), pp. 52–59. DOI: 10.51173/jt.v1i1.70. DOI:

Sarah Luanne Silva et al. “Phytochemical analysis and evaluation of the antimicrobial and antioxidant activities of extracts and fractions of Hymenaea eriogyne Benth”. Nat Prod Res 35(17) (2021), pp. 2937–2941. DOI: 10.1080/14786419.2019.1675066. DOI:

So Youn Park et al. “Concurrent treatment with taxifolin and cilostazol on the lowering of β-amyloid accumulation and neurotoxicity via the suppression of P-JAK2/P-STAT3/NF-κB/BACE1 signaling pathways”. PLoS One 11(12) (2016), e0168286–e0168286. DOI: 10.1371/journal.pone.0168286. DOI:

Suhail Razak et al. “Taxifolin, a natural flavonoid interacts with cell cycle regulators causes cell cycle arrest and causes tumor regression by activating Wnt/ β -catenin signaling pathway”. BMC Cancer 18 (2018), pp. 1043–1043. DOI: 10.1186/s12885-018-4959-4. DOI:

T G Borovskaya et al. “Dihydroquercetin effects on the morphology and antioxidant/prooxidant balance of the prostate in rats with sulpiride-induced benign hyperplasia”. Bull Exp Biol Med 185(4) (2015), pp. 513–516. DOI: 10.1007/s10517-015-2797-9. DOI:

Venkata Rao Krishnamurthi et al. “A new analysis method for evaluating bacterial growth with microplate readers”. PLoS One 16(1) (2021), e0245205–e0245205. DOI: 10.1371/journal.pone.0245205. DOI:

W Haque, S P Pattanayak, and B N Sinha. “Evaluation of taxifolin and phloretin as antiangiogenic flavonoids: An in vivo, in vitro experimental analysis”. Int J Pharm Pharm Sci 7 (2015), pp. 72–79.

Xin Chen et al. “Plant flavonoid taxifolin inhibits the growth, migration and invasion of human osteosarcoma cells”. Mol Med Rep 17(2) (2018), pp. 3239–3245. DOI: 10.3892/mmr.2017.8271. DOI:




How to Cite

Antibacterial and antibiofilm activities of taxifolin against vancomycin-resistant S. aureus (VRSA). (2022). Baghdad Journal of Biochemistry and Applied Biological Sciences, 3(04), 262-272.