Effects of acute inflammation on platelet indices: An experimental study

Veysel Tahiroglu; Fatih Kara

doi:10.47419/bjbabs.v3i03.150

Authors

Veysel Tahiroglu Department of Nursing, Faculty of Health Sciences, University of Sirnak, Sirnak, Turkey
Fatih Kara Department of Biochemistry, Faculty of Medicine, University of Kafkas, Kars, Turkey

DOI:

https://doi.org/10.47419/bjbabs.v3i03.150

Keywords:

acute inflammation, blood platelets, carrageenan, Mean platelet volume, rats

Abstract

Background and objective: It is well known that inflammation may affect the platelets. However, there are inconsistencies between the results of observational studies investigating changes in platelet indices in inflammatory conditions. This study aimed to investigate the possible effects of acute inflammation on platelet indices in plantar inflammation model in rats.

Methods: A total of 10 rats, 5 in each group, were used in the study. Lambda-carrageenan and saline were applied subcutaneously to the right hind paw of the rats in the inflammation group and in the control group, respectively. Six hours after the administration, blood samples were taken from femoral arteries and femoral veins, and platelet indices were measured by a hematology analyzer. In addition, plantar tissue samples belonging to the control and inflammation groups were evaluated histopathologically.

Results: On histopathological examination, no pathological condition was observed in the control group, while there were changes consistent with acute inflammation in the lambda-carrageenan-injected group. There was no significant difference in terms of platelet indices between both the arterial and vein samples and between the control and inflammation groups.

Conclusions: Our results suggest that platelet indices cannot be used in the diagnosis of acute inflammatory conditions. However, in our opinion, these findings must not be interpreted as that acute inflammation does not affect platelet number and volume. Instead, we believe that it may be more appropriate to say that acute inflammation does not produce a quantitatively significant change in platelet indices due to the combination of the opposite effects.

Metrics

Metrics Loading ...

Downloads

Download data is not yet available.

References

A Aydogan, S Akkucuk, S Arica, et al. “The analysis of mean platelet volume and platelet distribution width levels in appendicitis”. Indian J Surg 77(2) (2015), pp. 495–500. DOI: 10.1007/s12262-013-0891-7.

A J Bowdler. The complete spleen: structure, function, and clinical disorders. 2nd ed. USA: Springer Science & Business Media, 2001, pp. 110–112.

A Lipinska et al. “Complex calculation or quick glance? Mean platelet volume - new predictive marker for pulmonary embolism”. Ther Clin Risk Manag 14 (2018), pp. 2221–2228. DOI: 10.2147/TCRM.S181381.

A Seker, A ˙Incebiyik, A Kucuk, et al. “Mean platelet volume in patients with acute and chronic cholecystitis”. Acta Med Mediterr 29(3) (2013), pp. 515–519.

A T Sayit, P H Gunbey, and Y Terzi. “Is the mean platelet volume in patients with acute cholecystitis an inflammatory marker?” J Clin Diagn Res 9(6) (2015), TC05–TC07. DOI: 10.7860/JCDR/2015/12028.6061.

A Temizi et al. “Role of complete blood counts parameters in diagnosıs of acute cholecystitis”. Acta Medica Mediterr 33(3) (2017), pp. 411–416.

A Y Gasparyan et al. “Mean platelet volume: a link between thrombosis and inflammation?” Curr Pharm Des 17(1) (2011), pp. 47–58. DOI: 10.2174/138161211795049804.

B Ceylan et al. “Can platelet indices be used as predictors of complication in subjects with appendicitis?” Wien Klin Wochenschr 128(8) (2016), pp. 620–625. DOI: 10.1007/s00508-015-0760-4.

B Dinc, A Oskay, et al. “New parameter in diagnosis of acute appendicitis: platelet distribution width”. World J Gastroenterol 21(6) (2015), pp. 1821–1826. DOI: 10.3748/wjg.v21.i6.1821.

B Shah et al. “Comparison of platelet activity measurements by use of arterial and venous blood sampling”. J Thromb Haemost 11(10) (2013), pp. 1922–1924. DOI: 10.1111/jth.12370.

C A Winter, E A Risley, and G W Nuss. “Carrageenin-induced edema in hind paw of the rat as an assay for antiiflammatory drugs”. Proc Soc Exp Biol Med 111 (1962), pp. 544–547. DOI: 10.3181/00379727-111-27849.

H Erdem, R Aktimur, S Cetinkunar, et al. “Evaluation of mean platelet volume as a diagnostic biomarker in acute appendicitis”. Int J Clin Exp Med 8(1) (2015), pp. 1291–1295.

H G Downie et al. “Platelets and blood coagulation in arterial and venous blood”. Am J Physiol 205(5) (1963), pp. 982–984. DOI: 10.1152/ajplegacy.1963.205.5.982.

H Narci et al. “The role of mean platelet volume in the diagnosis of acute appendicitis: a retrospective case-controlled study”. Iran Red Crescent Med J 15(12) (2013), e11934–e11934. DOI: 10.5812/ircmj.11934.

J - J Lei et al. “Can mean platelet volume play a role in evaluating the severity of acute pancreatitis?” World J Gastroenterol 23(13) (2017), pp. 2404–2413. DOI: 10.3748/wjg.v23.i13.2404.

J - Y Noh. “Megakaryopoiesis and platelet biology: Roles of transcription factors and emerging clinical implications”. Int J Mol Sci 22(17) (2021), pp. 9615–9615. DOI: 10.3390/ijms22179615.

J Bester and E Pretorius. “Effects of IL-1β, IL-6 and IL-8 on erythrocytes, platelets and clot viscoelasticity”. Sci Rep 6 (2016), pp. 32188–32188. DOI: 10.1038/srep32188.

K Kaushansky. “Thrombopoiesis”. Semin Hematol 52(1) (2015), pp. 4–11. DOI: 10.1053/j.seminhematol.2014.10.003.

K Pogorzelska et al. “Characteristics of platelet indices and their prognostic significance in selected medical condition - a systematic review”. Adv Med Sci 65(2) (2020), pp. 310–315. DOI: 10.1016/j.advms.2020.05.002.

K Stark and S Massberg. “Interplay between inflammation and thrombosis in cardiovascular pathology”. Nat Rev Cardiol 18(9) (2021), pp. 666–682. DOI: 10.1038/s41569-021-00552-1.

L M Tocantins. “The mammalian blood platelet in health and disease”. Medicine 17 (1938), pp. 155–260.

M Gawlita et al. “Mean platelet volume and platelet-large cell ratio as prognostic factors for coronary artery disease and myocardial infarction”. Folia Cardiol 10(6) (2016), pp. 418–422. DOI: 10.5603/FC.2015.0079.

M Panova-Noeva, A Schulz, M I Hermanns, et al. “Sex-specific differences in genetic and nongenetic determinants of mean platelet volume: results from the Gutenberg Health Study”. Blood 127(2) (2016), pp. 251–259. DOI: 10.1182/blood-2015-07-660308.

P Noris, F Melazzini, and C L Balduini. “New roles for mean platelet volume measurement in the clinical practice?” Platelets 27(7) (2016), pp. 607–612. DOI: 10.1080/09537104.2016.1224828.

S Bilici et al. “Mean platelet volume in diagnosis of acute appendicitis in children”. Afr Health Sci 11(3) (2011), pp. 427–432.

S Danese, Carol de la Motte Cd, and C Fiocchi. “Platelets in inflammatory bowel disease: clinical, pathogenic, and therapeutic implications”. Am J Gastroenterol 99(5) (2004), pp. 938–945. DOI: 10.1111/j.1572-0241.2004.04129.x.

S Handtke and T Thiele. “Large and small platelets-(When) do they differ?” J Thromb Haemost 18(6) (2020), pp. 1256–1267. DOI: 10.1111/jth.14788.

S Margetic. “Inflammation and haemostasis”. Biochem medica 22(1) (2012), pp. 49–62.

S Morello et al. “Thrombo-Inflammation: A Focus on NTPDase1/CD39”. Cells 10(9) (2021), pp. 2223–2223. DOI: 10.3390/cells10092223.

T Tullavardhana, S Sanguanlosit, and A Chartkitchareon. “Role of platelet indices as a biomarker for the diagnosis of acute appendicitis and as a predictor of complicated appendicitis: A meta-analysis”. Ann Med Surg 66 (2021), pp. 102448–102448. DOI: 10.1016/j.amsu.2021.102448.

V Cortin et al. “Efficient in vitro megakaryocyte maturation using cytokine cocktails optimized by statistical experimental design”. Exp Hematol 33(10) (2005), pp. 1182–1191. DOI: 10.1016/j.exphem.2005.06.020.

Y Albayrak, A Albayrak, F Albayrak, et al. “Mean platelet volume: a new predictor in confirming acute appendicitis diagnosis”. Clin Appl Thromb Hemost 17(4) (2011), pp. 362–366. DOI: 10.1177/1076029610364520.

Y Beyazit, A Sayilir, S Torun, et al. “Mean platelet volume as an indicator of disease severity in patients with acute pancreatitis”. Clin Res Hepatol Gastroenterol 36(2) (2012), pp. 162–168. DOI: 10.1016/j.clinre.2011.10.003.

Y Yilmaz et al. “The platelet indices in pediatric patients with acute appendicitis”. Int J Res Med Sci 3(6) (2015), pp. 1388–1391. DOI: 10.18203/2320-6012.ijrms20150153.

Z Fan et al. “Mean platelet volume and platelet distribution width as markers in the diagnosis of acute gangrenous appendicitis”. Dis Markers 2015 (2015), pp. 542013–542013. DOI: 10.1155/2015/542013.

Z W Yang et al. “Comparison of blood counts in venous, fingertip and arterial blood and their measurement variation”. Clin Lab Haematol 23(3) (2001), pp. 155–159. DOI: 10.1046/j.1365-2257.2001.00388.x.