Safety assessment and biochemical evaluation of the effect of biogenic silver nanoparticles (using bark extract of C. zeylanicum) on Rattus norvegicus rats
Safety of biofabricated AgNPs (using Cinnamomum zeylanicum extract)
Keywords:cinnamon, green synthesis, histopathology, oxidative stress, silver nanoparticles, subacute toxicity
Background: Biosynthesized silver nanoparticles (AgNPs) are widely used in various biomedical applications. However, limited reports are currently available about the safety of biofabricated AgNPs using Cinnamomum zeylanicum bark extracts.
Objectives: The current study is aimed to assess the potential toxicity of biosynthesized AgNPs (using C. zeylanicum) by subacute oral administration in experimental rats.
Methods: AgNPs were biofabricated using methanol extract of C. zeylanicum bark and characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM). Twenty-four Rattus norvegicus female rats were divided into 4 groups (6 animals per group) as follows: Group-I was the control, while groups II, III, and IV were given 0.85, 1.76 and 3.53 mg/kg doses of AgNPs, respectively for 14 consecutive days. After 14 days of oral administration of AgNPs, serum levels of malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) were measured using an ELISA technique. Serum concentrations of urea, creatinine, aspartate transaminase (AST) and alanine transaminase (ALT) were also determined as well as histopathologic features of the liver and the kidney.
Results: AgNPs did not induce any changes in mean body weight, biochemical parameters (AST, ALT, urea and creatinine), oxidative stress biomarkers (MDA, SOD and CAT) and histopathologic features (of the liver and kidneys) of the treated groups when compared to control group.
Conclusions: Our findings suggest that the oral administration of biogenic AgNPs (biofabricated using methanol extract of C. zeylanicum) to rats at a specific dose is relatively safe and does not show any signs of toxicity.
A Leela and M Vivekanandan. “Tapping the unexploited plant resources for the synthesis”. Afr J Biotechnol 7 (2008), pp. 3162–3165.
Antonio Ayala, Mario F. Muñoz, and Sandro Argüelles. “Lipid Peroxidation: Production, Metabolism, and Signaling Mechanisms of Malondialdehyde and 4-Hydroxy-2-Nonenal”. Oxid Med Cell Longev 2014 (2014), pp. 1–31. DOI: 10.1155/2014/360438.
Asmaa Mohamed El Shafey. “Green synthesis of metal and metal oxide nanoparticles from plant leaf extracts and their applications: A review”. Green Process Synth 9(1) (2020), pp. 304–339. DOI: 10.1515/gps-2020-0031.
E Swanson and W D Wallace. “Methods in Molecular Biology”. In: Histopathology Methods and Protocols. Ed. by Christina E. Day et al. Vol. 1180. Methods in Molecular Biology. Humana Press, New York, NY, 2014, pp. 283–291. DOI: 10.1007/978-1-4939-1050-2.
G A Nasir, H F Samir, and A K Mohammed. “Effect of silver Nanoparticals in Liver Function Enzymes and Oxidative Stress Levels”. World J Pharm Res 4 (2015), pp. 2080–2089.
G Kumararaja, R Sundaraganapathy, and I Constantine. “Comparative studies on synthesized silver nanoparticles using Artemisia vulgaris Linn., and Cinnamomum zeylanicum Nees., for their antifungal activity”. J Pharm Sci Res 11 (2019), pp. 2558–2565.
G. Oberdörster. “Safety assessment for nanotechnology and nanomedicine: concepts of nanotoxicology”. J Intern Med 267(1) (2010), pp. 89–105. DOI: 10.1111/j.1365-2796.2009.02187.x.
Gurpreet Singh Dhillon et al. “Green approach for nanoparticle biosynthesis by fungi: current trends and applications”. Crit Rev Biotechnol 32(1) (2012), pp. 49–73. DOI: 10.3109/07388551.2010.550568.
Hyman J. Zimmerman. “Drug-Induced Liver Disease”. Drugs 16 (1978), pp. 25–45. DOI: 10.2165/00003495-197816010-00002.
Idris Bello et al. “Acute and Sub-Acute Toxicity Evaluation of the Methanolic Extract of Alstonia scholaris Stem Bark”. Med Sci 4(1) (2016), pp. 4–4. DOI: 10.3390/medsci4010004.
J M Anderson and Biocompatibility. “Biocompatibility”. In: Polymer Science: A Comprehensive Reference. Ed. by and others. Vol. 10. Elsevier, June 2012, pp. 363–383. DOI: 10.1016/B978-0-444-53349-4.00229-6.
K Aamir, H U Khan, and C F Hossain. “Oral toxicity of arjunolic acid on hematological, biochemical and histopathological investigations in female Sprague Dawley rats”. PeerJ 2019 (2019), pp. 1–24. DOI: 10.7717/peerj.8045.
Koffi KOUAME et al. “Effect of long-term administration of Cinnamomum cassia silver nanoparticles on organs (kidneys and liver) of Sprague-Dawley rats”. Turk J Biol 42(6) (2018), pp. 498–505. DOI: 10.3906/biy-1805-103.
Muhammad Ashraf Sabri et al. “Selection of Suitable Biological Method for the Synthesis of Silver Nanoparticles”. Nanomater Nanotechnol 6 (2016), pp. 29–29. DOI: 10.5772/62644.
Munnaluri Gauthami et al. “Synthesis of Silver Nanoparticles using Cinnamomum zeylanicum Bark Extract and its Antioxidant Activity”. Nanosci Nanotechnol - Asia 5(1) (2015), pp. 2–7. DOI: 10.2174/221068120501150728103209.
Nabaa A. Jasim et al. “ZnO nanoparticles inhibit growth and biofilm formation of vancomycin-resistant S. aureus (VRSA)”. Biocatal Agric Biotechnol 29 (2020), pp. 101745–101745. DOI: 10.1016/j.bcab.2020.101745.
Osamudiamen Ebohon, Francis Irabor, and Ehimwenma Sheena Omoregie. “Sub-acute toxicity study of methanol extract of Tetrorchidium didymostemon leaves using biochemical analyses and gene expression in Wistar rats”. Heliyon 6(6) (2020), e04313–e04313. DOI: 10.1016/j.heliyon.2020.e04313.
Péter Bélteky et al. “Silver nanoparticles: aggregation behavior in biorelevant conditions and its impact on biological activity”. Int J Nanomed Volume 14 (2019), pp. 667–687. DOI: 10.2147/ijn.s185965.
R W Rajesh, L R Jaya, and K S Niranjan. “Phytosynthesis of Silver Nanoparticle Using Gliricidia sepium (Jacq.)” Curr Nanosci 5 (2009), pp. 117–122. DOI: 10.2174/157341309787314674.
Renu Sankar et al. “Anticancer activity of Ficus religiosa engineered copper oxide nanoparticles”. Mater Sci Eng C 44 (2014), pp. 234–239. DOI: 10.1016/j.msec.2014.08.030.
Rosa Linares et al. “In Adult Rats With Polycystic Ovarian Syndrome, Unilateral or Bilateral Vagotomy Modifies the Noradrenergic Concentration in the Ovaries and the Celiac Superior Mesenteric Ganglia in Different Ways”. Front Physiol 10 (2019), pp. 1–9. DOI: 10.3389/fphys.2019.01309.
S Bhardwaj and D Gupta. “Study of acute, Sub acute and chronic toxicity test”. Int J Curr Pharm Res 2 (2012), pp. 103–129.
S Kim and D Y Ryu. “Silver nanoparticle-induced oxidative stress, genotoxicity and apoptosis in cultured cells and animal tissues”. J Appl Toxicol 33 (2013), pp. 78–89. DOI: 10.1002/jat.2792.
S M Yaseen et al. “Antibacterial activity of palm heart extracts collected from Iraqi Phoenix dactylifera L”. J Tech 1 (2020), pp. 52–59. DOI: 10.51173/jt.v1i1.70.
Sabah Ansar et al. “Eco friendly silver nanoparticles synthesis by Brassica oleracea and its antibacterial, anticancer and antioxidant properties”. Sci Rep 10(1) (2020), pp. 1–12. DOI: 10.1038/s41598-020-74371-8.
Sameer Mohammed Yaseen, Hussein Adil Abid, and Mudhar Abdul-Wahab Al-Obaidi. “Disturbed levels of non-enzymatic antioxidants and malondialdehyde among makeup users”. J Tech 2(1) (2020), pp. 42–48. DOI: 10.51173/jt.v2i1.166.
V Lobo et al. “Free radicals, antioxidants and functional foods: Impact on human health”. Pharmacogn Rev 4(8) (2010), pp. 118–118. DOI: 10.4103/0973-7847.70902.
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